Intracellular murine hepatitis virus-specific RNAs contain common sequences
Identifieur interne : 001E58 ( Main/Exploration ); précédent : 001E57; suivant : 001E59Intracellular murine hepatitis virus-specific RNAs contain common sequences
Auteurs : Steve Cheley [Canada] ; Robert Anderson [Canada] ; Margaret J. Cupples [Canada] ; Edwin C. M. Lee Chan [Canada] ; Vincent L. Morris [Canada]Source :
- Virology [ 0042-6822 ] ; 1981.
English descriptors
- Teeft :
- Acad, Actinomycin, Annealing, Aqueous phase, Avian coronavirus, Bronchitis, Bronchitis virus, Cdna, Cdna annealed, Cell monolayers, Cheley, Coronavirus, Coronavirus multiplication strategy, Dalton, Ethanol, Final concentration, Hybridization, Infectious bronchitis virus, Irlfected cells, Major polyadenylated, Molecular weights, Mrna, Murine, Murine coronavirus, Murine hepatitis, Murine hepatitis virus, Nonstructural polypeptides, Nucleic acids, Nucleocapsid protein, Petri dishes, Phosphate buffer, Polyadenylated, Polypeptide, Potassium acetate, Proc, Protein synthesis, Ribonucleic acid, Rna, Room temperature, Semliki forest virus, Several hours, Structural polypeptides, Structural protein, Structural proteins, Subgenomic rnas, Translation products, Translation system, Translational initiation, Tumor virus, Uninfected, Viral, Viral nucleocapsid protein, Viral proteins, Viral rnas, Virol, Wege, Western ontario.
Abstract
Abstract: A major polyadenylated viral RNA of approximately 0.8 × 106 daltons was isolated from murine hepatitis virus (A59)-infected cells by preparative polyacrylamide gel electrophoresis in formamide. This RNA was shown to encode the viral nucleocapsid protein by direct in vitro translation in a cell-free, reticulocyte-derived system. Single stranded 32P-labeled complementary DNA was prepared from this RNA and was demonstrated to be virus specific. Using this complementary DNA in a Northern blotting procedure, we were able to identify six major virus-specific intracellular RNA species with estimated molecular weights of 0.8, 1.1, 1.4, 1.6, 3, and 4 × 106 daltons. All of these RNA species were polyadenylated. Our results support the idea that coronavirus-infected cells contain multiple intracellular polyadenylated RNAs which share common sequences.
Url:
DOI: 10.1016/0042-6822(81)90305-6
Affiliations:
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Cupples</name><affiliation wicri:level="1"><country xml:lang="fr">Canada</country><wicri:regionArea>Department of Microbiology and Immunology, University of Western Ontario, London, Ontario N6A 5C1</wicri:regionArea><wicri:noRegion>Ontario N6A 5C1</wicri:noRegion></affiliation></author><author><name sortKey="Lee Chan, Edwin C M" sort="Lee Chan, Edwin C M" uniqKey="Lee Chan E" first="Edwin C. M." last="Lee Chan">Edwin C. M. Lee Chan</name><affiliation wicri:level="1"><country xml:lang="fr">Canada</country><wicri:regionArea>Department of Microbiology and Immunology, University of Western Ontario, London, Ontario N6A 5C1</wicri:regionArea><wicri:noRegion>Ontario N6A 5C1</wicri:noRegion></affiliation></author><author><name sortKey="Morris, Vincent L" sort="Morris, Vincent L" uniqKey="Morris V" first="Vincent L." last="Morris">Vincent L. Morris</name><affiliation wicri:level="1"><country xml:lang="fr">Canada</country><wicri:regionArea>Department of Microbiology and Immunology, University of Western Ontario, London, Ontario N6A 5C1</wicri:regionArea><wicri:noRegion>Ontario N6A 5C1</wicri:noRegion></affiliation></author></analytic><monogr></monogr><series><title level="j">Virology</title><title level="j" type="abbrev">YVIRO</title><idno type="ISSN">0042-6822</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1981">1981</date><biblScope unit="volume">112</biblScope><biblScope unit="issue">2</biblScope><biblScope unit="page" from="596">596</biblScope><biblScope unit="page" to="604">604</biblScope></imprint><idno type="ISSN">0042-6822</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0042-6822</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="Teeft" xml:lang="en"><term>Acad</term><term>Actinomycin</term><term>Annealing</term><term>Aqueous phase</term><term>Avian coronavirus</term><term>Bronchitis</term><term>Bronchitis virus</term><term>Cdna</term><term>Cdna annealed</term><term>Cell monolayers</term><term>Cheley</term><term>Coronavirus</term><term>Coronavirus multiplication strategy</term><term>Dalton</term><term>Ethanol</term><term>Final concentration</term><term>Hybridization</term><term>Infectious bronchitis virus</term><term>Irlfected cells</term><term>Major polyadenylated</term><term>Molecular weights</term><term>Mrna</term><term>Murine</term><term>Murine coronavirus</term><term>Murine hepatitis</term><term>Murine hepatitis virus</term><term>Nonstructural polypeptides</term><term>Nucleic acids</term><term>Nucleocapsid protein</term><term>Petri dishes</term><term>Phosphate buffer</term><term>Polyadenylated</term><term>Polypeptide</term><term>Potassium acetate</term><term>Proc</term><term>Protein synthesis</term><term>Ribonucleic acid</term><term>Rna</term><term>Room temperature</term><term>Semliki forest virus</term><term>Several hours</term><term>Structural polypeptides</term><term>Structural protein</term><term>Structural proteins</term><term>Subgenomic rnas</term><term>Translation products</term><term>Translation system</term><term>Translational initiation</term><term>Tumor virus</term><term>Uninfected</term><term>Viral</term><term>Viral nucleocapsid protein</term><term>Viral proteins</term><term>Viral rnas</term><term>Virol</term><term>Wege</term><term>Western ontario</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Abstract: A major polyadenylated viral RNA of approximately 0.8 × 106 daltons was isolated from murine hepatitis virus (A59)-infected cells by preparative polyacrylamide gel electrophoresis in formamide. This RNA was shown to encode the viral nucleocapsid protein by direct in vitro translation in a cell-free, reticulocyte-derived system. Single stranded 32P-labeled complementary DNA was prepared from this RNA and was demonstrated to be virus specific. Using this complementary DNA in a Northern blotting procedure, we were able to identify six major virus-specific intracellular RNA species with estimated molecular weights of 0.8, 1.1, 1.4, 1.6, 3, and 4 × 106 daltons. All of these RNA species were polyadenylated. Our results support the idea that coronavirus-infected cells contain multiple intracellular polyadenylated RNAs which share common sequences.</div></front></TEI><affiliations><list><country><li>Canada</li></country></list><tree><country name="Canada"><noRegion><name sortKey="Cheley, Steve" sort="Cheley, Steve" uniqKey="Cheley S" first="Steve" last="Cheley">Steve Cheley</name></noRegion><name sortKey="Anderson, Robert" sort="Anderson, Robert" uniqKey="Anderson R" first="Robert" last="Anderson">Robert Anderson</name><name sortKey="Cupples, Margaret J" sort="Cupples, Margaret J" uniqKey="Cupples M" first="Margaret J." last="Cupples">Margaret J. Cupples</name><name sortKey="Lee Chan, Edwin C M" sort="Lee Chan, Edwin C M" uniqKey="Lee Chan E" first="Edwin C. M." last="Lee Chan">Edwin C. M. Lee Chan</name><name sortKey="Morris, Vincent L" sort="Morris, Vincent L" uniqKey="Morris V" first="Vincent L." last="Morris">Vincent L. Morris</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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